Figure 9
From: The molecular basis of defective lens development in the Iberian mole
TUNEL assay in mouse and mole lenses. Terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labelling (TUNEL)-positive cells are observed in the lens core (LC) but not in the lens epithelium (LE) of 18 days post-coitum mice embryos (E18). In contrast, TUNEL reaction was detected in most of the epithelial cells but not in the lens fibres of infant moles from the s10 stage. The nuclei are stained blue with 4'6-diamidino-2-phenylindole stain. Haematoxylin photomicrographs from the same sections used in TUNEL analysis are shown in the bottom row at lower magnifications to assess lens structure. Scale bar represents 100 ÎĽm in the immunofluorescence photomicrographs and 400 ÎĽm in the haematoxylin-stained sections.