Inducible and constitutive heat shock gene expression responds to modification of Hsp70 copy number in Drosophila melanogaster but does not compensate for loss of thermotolerance in Hsp70null flies

Table 2 F-statistics and statistical significance of mixed model ANOVAs of gene expression

Gene	Time	Strain	Treatment	Strain-by-treatment	RpL32
GstE1	0	13.27****	3.25*	3.15**	12.36***
	1	6.05**	11.42****	6.04****	8.13**
Hsc70_1	0	†	†	†	†
	1	†	†	†	†
Hsc70_2	0	9.79****	9.96****	4.39****	27.36****
	1	7.74***	18.63****	7.11****	6.58*
Hsc70_3	0	2.4	11.44****	12.8****	20.82****
	1	6.28**	53.46****	11.45****	14.68***
Hsc70_4	0	7.71***	33.5****	17.83****	25.62****
	1	6.66***	51.26****	20.17****	21.47****
Hsc70_5	0	4.42**	17.69****	7.08****	27.46****
	1	10.84****	34.83****	13.34****	17.76****
Hsp22	0	8.32***	50.25****	7.89****	7.77**
	1	4.41**	75.47****	11.81****	9**
Hsp23	0	4.33**	17.26****	7.92****	7.37**
	1	10.41****	50.16****	18.68****	7.77**
Hsp26	0	2.78	39.13****	4.38****	14.83***
	1	1.32	45.33****	7.97****	1.9
Hsp27	0	2.32	14.5****	10.7****	24.39****
	1	3.77*	52.41****	17.26****	20.94****
Hsp40	0	2.12	25.39****	13.65****	19.15****
	1	3.22*	70.16****	23.96****	11.17**
Hsp60	0	3.26*	28.6****	14.89****	0.64
	1	20.72****	81.57****	19.84****	19.25****
Hsp67Ba	0	1.23	2.05	1.76	25.24****
	1	0.83	4.62**	2.11*	17.54****
Hsp67Bb	0	‡	‡	‡	‡
	1	‡	‡	‡	‡
Hsp67Bc	0	2.16	11.82****	7.37****	15.99***
	1	4.44	38.33****	12.58****	25.98****
Hsp68	0	1.3	45.67****	12.28****	45.1****
	1	3.02*	82.17****	18.6****	30.48****
Hsp70	0	49.74****	43.36****	11.57****	11.84**
	1	70.62****	89.25****	13.64****	4.2*
Hsp83	0	1.19	16.64****	12.54****	28.63****
	1	4.44**	53.35****	14.05****	3.41

'Gene' and 'Time' indicate gene examined and time following treatment, respectively. Remaining columns indicate F-statistics associated with effects of Strain (df = 3), Treatment (df = 5), strain-by-treatment interaction (df = 15), and expression of the RpL32 control gene (covariate; df = 1). Significance levels: ****, P < 0.0001; ***, P < 0.001; **, P < 0.01; *, P < 0.05. Cells without asterisks indicate statistically insignificant values. † indicates that Hsc70-1 was not amplified with sufficient efficiency to calculate a standard curve and was excluded from analysis. ‡ indicates that Hsp67Bb was not amplified with sufficient specificity and was excluded from analysis.

ISSN: 1741-7007