Figure 1
From: An internal ribosome entry site element directs the synthesis of the 80 kDa isoforms of protein 4.1R
Analysis of the protein products synthesized in vitro from 4.1R cDNAs. A. Schematic representation of the exon map for the 4.1R protein. Exons are coded as follows: striped, alternative; white, constitutive; black, non-coding. The number of each exon is shown at the bottom. Two translation-initiation sites at exons 2' (ATG1) and exon 4 (ATG2) are indicated, as is the stop codon (TGA) at exon 21. B and C, exon map of the 4.1R135 cDNAs (B) and the 4.1R80 cDNAs (C) used in the in vitro-coupled transcription and translation assays. D. In vitro-coupled transcription and translation reactions of the indicated 4.1R cDNAs. Reaction products were labelled with [35S] methionine and autoradiographed. A control reaction containing all components of the mixture except the cDNA template is also shown (-).