Figure 3
From: An internal ribosome entry site element directs the synthesis of the 80 kDa isoforms of protein 4.1R
The short protein 4.1R is also expressed in vivo from 4.1R 135 cDNAs. A. Western blot of total protein extracts of COS-7 cells transfected with an empty plasmid (-) or transfected with 4.1R cDNAs 1' and 1 revealed with anti-myc (anti-myc) and anti-FLAG (anti-FLAG) antibodies. B. Western blot of total protein extracts of COS-7 cells transfected with an empty plasmid (-) or transfected with 4.1R cDNAs 4' and 4 revealed with anti-myc and anti-FLAG antibodies. All 4.1R proteins were tagged at the amino and carboxyl termini with FLAG and myc epitopes, respectively, to detect the exogenously expressed proteins 4.1R.