Skip to main content
Figure 5 | BMC Biology

Figure 5

From: An internal ribosome entry site element directs the synthesis of the 80 kDa isoforms of protein 4.1R

Figure 5

The 4.1R sequence upstream of the ATG2 directs translation of the second cistron from bicistronic vectors. A. Schematic representation of the bicistronic and monocistronic constructs used. B. The indicated constructs were transfected into COS-7 cells. Luciferase activity or fluorescence intensity was determined when the bicistronic vectors containing the Renilla/firefly luciferase or the DsRed/EGFP reporters, respectively, were used. The firefly:Renilla or EGFP:DsRed ratios are expressed relative to that of the plasmids containing the sequence of 4.1R in the antisense orientations (RLuc-4.1a-FLuc and DsRed-4.1a-EGFP, respectively), which were assigned a value of 1. Error bars correspond to the SEM from five independent experiments. C. Total RNA was isolated from COS-7 cells transfected with an empty plasmid (-) or transfected with DsRed-4.1s-EGFP, DsRed-4.1a-EGFP and pEGFP and analyzed by Northern blot using full-length EGFP cDNA as a hybridization probe. The arrowhead indicates the position of the full-length bicistronic RNA and the asterisk that of the EGFP monocistronic RNA.

Back to article page