Figure 6
From: An internal ribosome entry site element directs the synthesis of the 80 kDa isoforms of protein 4.1R
The 4.1R sequence contains an IRES element driving translation of the second cistron. A. Schematic representations of the two bicistronic constructs used in which a stable hairpin (
) that impedes ribosome scanning was cloned upstream (HDsRed-4.1s-EGFP) or downstream (DsRedH-4.1s-EGFP) of the first cistron, DsRed. B. The indicated constructs were transfected into COS-7 cells and their fluorescence intensity determined. Values presented are normalized against the fluorescence intensity produced from the plasmid DsRed-4.1s-EGFP, which was assigned a value of 100. Error bars correspond to the SEM from four experiments.