Figure 5
Activated cPLA 2 is associated with PrP Sc in ScGT1 cells. (A) Immunoblots showing amounts of cPLA2 immunoprecipitated with antibodies to PrP (4F2 or IC18), CD55 or an isotype control (Iso) from untreated ScGT1 cells (Untreated) or from ScGT1 cells pre-treated with 0.2 units PI-PLC for 30 minutes (PI-PLC). (B) The amounts of activated cPLA2 (expressed as a percentage) in whole cell extracts from ScGT1 cells (open bars), from ScGT1 cell extracts depleted with the anti-PrP mab 4F2 (black bars) and in 4F2 immunoprecipitates (IPT) from ScGT1 cell extracts (striped bars). Values shown are the mean ± SD from 10 samples.