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Table 2 Correlation between PrPSc and free cholesterol content in ScGT1 cells. The amounts of PrPSc and free cholesterol in ScGT1 and GT1 cells treated for 7 days with neurotrophic factors or retinoic acid as shown. Free cholesterol was measured in cell extracts using the Amplex Red cholesterol assay kit. Values shown are the mean ± SD from 9 samples. PrPSc content of cells significantly greater than those of untreated cells (P < 0.05) are indicated by * and free cholesterol content of cells significantly greater than those of untreated cells (P < 0.05) are indicated by #.

From: Sequestration of free cholesterol in cell membranes by prions correlates with cytoplasmic phospholipase A2activation

  PrPSc (ng/ml) Free cholesterol (ng/mg protein)
  ScGT1 GT1 ScGT1 GT1
Control 6.9 ± 0.2 - 510 ± 48 392 ± 44
10 ng/ml BDNF 15.2 ± 1.4* - 654 ± 61# 417 ± 43
10 ng/ml GDNF 14.2 ± 0.5* - 655 ± 59# 393 ± 32
10 ng/ml NGF 7.4 ± 1.7 - 503 ± 72 364 ± 45
100 nM retinoic acid 16.5 ± 2.1* - 705 ± 83# 440 ± 59