Table 2 Correlation between PrPSc and free cholesterol content in ScGT1 cells. The amounts of PrPSc and free cholesterol in ScGT1 and GT1 cells treated for 7 days with neurotrophic factors or retinoic acid as shown. Free cholesterol was measured in cell extracts using the Amplex Red cholesterol assay kit. Values shown are the mean ± SD from 9 samples. PrPSc content of cells significantly greater than those of untreated cells (P < 0.05) are indicated by * and free cholesterol content of cells significantly greater than those of untreated cells (P < 0.05) are indicated by #.
| Â | PrPSc (ng/ml) | Free cholesterol (ng/mg protein) | ||
|---|---|---|---|---|
| Â | ScGT1 | GT1 | ScGT1 | GT1 |
Control | 6.9 ± 0.2 | - | 510 ± 48 | 392 ± 44 |
10 ng/ml BDNF | 15.2 ± 1.4* | - | 654 ± 61# | 417 ± 43 |
10 ng/ml GDNF | 14.2 ± 0.5* | - | 655 ± 59# | 393 ± 32 |
10 ng/ml NGF | 7.4 ± 1.7 | - | 503 ± 72 | 364 ± 45 |
100 nM retinoic acid | 16.5 ± 2.1* | - | 705 ± 83# | 440 ± 59 |