Skip to main content

Table 2 Effect of antibiotics on rate of mortality by B. thuringiensis (Bt) and Enterobacter sp. NAB3 (EntB) on six Lepidoptera species.

From: Contributions of gut bacteria to Bacillus thuringiensis-induced mortality vary across a range of Lepidoptera

  

LT25 (95% FL) a

LT50 (95% FL)

Species

Bacterial treatment

No antibiotics

Antibiotics

No antibiotics

Antibiotics

Vanessa cardui

untreated

no toxicity

no toxicity

no toxicity

no toxicity

 

Enterobacter

no toxicity

NAb

no toxicity

NA

 

Bt DiPel 25 IU

1.80 (1.54–2.26)

13.20 (7.83 – >100)*

1.91 (0.83–2.25)

29.48 (12.44 – >100)*

 

Bt DiPel 25 IU + EntB

NA

1.62 (1.24–1.95)#

NA

2.61 (2.21–2.97)#

Manduca sexta

untreated

no toxicity

no toxicity

no toxicity

no toxicity

 

Enterobacter

no toxicity

NA

no toxicity

NA

 

Bt DiPel 25 IU

1.89 (1.48–2.23)

no toxicity

3.04 (2.63–3.44)

no toxicity

 

Bt DiPel 25 IU + EntB

NA

3.81 (3.24–4.31)

NA

5.95 (5.23–7.16)

Pieris rapae

untreated

10.34 (7.36–806.32)

no toxicity

16.62 (9.67–>100)

No toxicity

 

Enterobacter

6.91 (5.21–14.02)

no toxicity

16.57 (9.79–96.47)

no toxicity

 

Bt DiPel 25 IU

1.26 (1.02–1.47)

12.95 (7.83–>100)*

1.76 (1.52–1.99)

27.38 (12.04–>100)*

 

Bt DiPel 25 IU + EntB

1.98 (1.66–2.27)#

1.47 (1.23–1.67)#

2.84 (2.52–3.16)#

1.98 (1.75–2.20)#

Heliothis virescens

untreated

no toxicity

NA

no toxicity

NA

 

Enterobacter

no toxicity

NA

no toxicity

NA

 

Bt DiPel 100 IU

0.48 (0.17–0.80)

8.62 (7.00–48.23)*

1.13 (0.80–1.77)

11.82 (8.48–267)*

 

Bt DiPel 100 IU + EntB

NA

no toxicity

NA

no toxicity

Pectinophora gossypiella

untreated

no toxicity

no toxicity

no toxicity

no toxicity

 

Enterobacter

no toxicity

no toxicity

no toxicity

no toxicity

 

Bt MVPII 10 μg

17.87 (15.48–20.22)

15.46 (13.44–16.68)

20.99 (18.94–29.59)

17.56 (16.21–19.20)

 

Bt MVPII 10 μg + EntB

7.11 (3.61–9.44)#

6.30 (2.78–8.62)#

13.71 (10.52–20.13)

12.61 (9.42–18.18)

Lymantria dispar

untreated

15.16 (8.90–398.16)

no toxicity

40.09 (16.05–>100)

no toxicity

 

Enterobacter

no toxicity

no toxicity

no toxicity

no toxicity

 

Bt MVPII 10 μg

4.97 (4.28–5.68)

9.52 (7.99–34.42)*

8.17 (6.95–9.41)

12.41 (10.40–125.70)*

 

Bt MVPII 10 μg + EntB

NA

5.23 (4.62–5.81)#

NA

7.62 (6.76–9.24)#

  1. Larval mortality rates were analyzed by PROC PROBIT. Estimates of the time (day) at which 25% and 50% of larvae died for each treatment are listed. Treatments with no significant mortality (X 2 > 0.05) are defined as having no toxicity. A cut-off of > 100 was assigned to upper FL estimates (note: these are computational outputs, not actual estimates of larval lifespan). Bt mortality rates denoted with * are significantly different (non-overlapping fiducial limits (FL)) with their respective antibiotic treatment. Values denoted with # are significantly different with the addition of Enterobacter sp. NAB3, as compared with Bt alone. a Units are days, b NA = Not tested