Cluster and annotation of JH-response genes. (A) Hierarchical clustering of individual butterflies (top) based on expression profiles of the 23 regulated genes expressed as heat maps (below). Animal ID code for clustering is as depicted in Table 1. Bootstrap analysis provides confidence values at the nodes. The heat maps show estimated gene expression levels (red upregulated; green downregulated). The genes have been ordered according to k-means clustering. (B) The sequence ID, annotation, and Biological Function based on gene ontology (GO) are shown below. They are listed in the same order they are represented on the vertical axis of panel A. NA means the gene is either not annotated or annotated but without GO function assigned.