Figure 3

Expression of the CaR in HEK293 cells decreases AUF1 and increases KSRP interaction with the PTH mRNA. RNA immunoprecipitation (RIP) analysis of extracts from cells transiently transfected with expression plasmids for PTH and GH as control and either the CaR plasmid or empty vector. Immunoprecipitation was performed using antibodies for AUF1, KSRP or control IgG. Input (A) and immunoprecipitiated (B, C) samples were analyzed by qPCR for PTH and GH mRNA. Results are presented as PTH mRNA corrected for GH mRNA. PTH mRNA in the immunoprecipitated samples was corrected for PTH mRNA in the input. The results are mean ± SE of three repeat experiments. *, P < 0.05 compared with cells transfected with empty vector (CaR-).