Actin caps and NBs are disrupted in hemizygous shibire mutant testes after heat pulse. (A), (B) Wild type (A) and hemizygous shi ts1mutant (B) testes labeled with FITC:phalloidin (green) and 4',6-diamidino-2-phenylindole (DAPI) (blue) after 30 minutes at 29°C. Regions populated by the coiled-up sperm bundles are marked by the dotted lines. Note that the base (arrows) is slightly enlarged in shi ts1hemizygous testis. Figures (b), (c) of (A) as well as (B) are enlarged views of the regions marked in (a) of (A), and (a) of (B), respectively. The figures in the (c) of A and (B) indicate the DAPI labeled NBs. The white arrowheads indicate actin caps and arrows indicate the NBs in respective figures. The red star indicates a highly disrupted NB and red arrowhead indicates abnormal actin caps in figures (b) and (c) of (B). (C) Isolated cysts stained with RITC: phalloidin (red) and DAPI (blue) from (a, b) wild type control and (c, d) the shi ts1hemizygous mutant testes before and after 30 minutes at 29°C. Arrows indicate actin caps in figures (c) and (d). (D) Histograms indicate average NBs in a testis in wild type and shi ts1adults after 30 minutes of incubation at 18°C (grey filled bars) and at 29°C (open bars), respectively. The error bars indicate ± S. E. M. The number of specimen (n) for each bar and the pair wise test of significance (p values) are indicated on each figure. The non-significant (ns), significant (*) and very significant (**) differences are indicated on each set of bars linked by the horizontal lines.