RBP4 expression in wild-type and Hmga1 -deficient mice. RBP4 mRNA in liver and fat from control and Hmga1-deficient mice, as measured by qRT-PCR (left), and densitometric quantification of RBP4 serum levels as detected by Western blot (WB) of serum samples (2 μl) from mice with diverse genotypes (right). In WB analysis, an anti-transthyretin (TTR) antibody was used to confirm similar amounts of protein on each lane. Results are from 4–6 mice in each group. *P < 0.01 versus control mice.