Hmga1 and RBP4 mRNA and protein expression in vivo , in glucagon-injected wild-type mice. Total RNA was isolated from liver (upper left) and fat (upper right) of 3-h-fasted mice, before and after intraperitoneal injection of glucagon. Levels of Hmga1 and RBP4 mRNA were measured at the indicated time intervals by qRT-PCR and normalized to RPS9 mRNA abundance, as described in Methods. Results are the mean values ± s.e.m. from 4–6 animals per group. Black bars, Hmga1 mRNA; gray bars, RBP4 mRNA. Representative Western blots from liver (lower left) and fat (lower right) of mice before and after glucagon injection are shown. Densitometric analyses of immunoblots are shown in bar graphs as the mean ± s.e.m. of data from 3–5 mice per each time point. Black bars, HMGA1; gray bars, RBP4.*P < 0.05 versus control mice (time 0).