Figure 9

Effects of RBP4 on insulin sensitivity. Insulin-tolerance test (ITT) was assessed in Hmga1-deficient mice injected with saline alone (left), and in Hmga1 mutants injected chronically with purified RBP4 (right) (n = 6–8 per genotype in each condition). ITT was performed by measuring blood glucose levels in 12-h-fasted conscious mice injected intraperitoneally with human insulin (Human Actrapid, Novo Nordisk), 1 U/kg body weight. Open squares, Hmga1 ^+/+; open circles, Hmga1 ^+/-; open diamonds, Hmga1 ^-/-. The degree of statistical significance was less in RBP4-injected Hmga1-deficient mice compared with the significance for saline-injected Hmga1 mutants. *P < 0.0001, saline-injected Hmga1-deficient mice versus Hmga1 ^+/+; # P < 0.05, RBP4-injected Hmga1 ^-/- mice versus Hmga1 ^+/+.