Figure 3

Microarray analysis validation for mouse embryo fibroblasts (MEFs). Microarray data (Additional file 2) were validated by quantitative real-time polymerase chain reaction (qRT-PCR) for nine genes on RNA extracted from three independent AP-2α +/- or -/- mouse embryo fibroblast (MEF) preparations. The 18S rRNA or GAPDH housekeeping genes were used as internal controls to normalize the data. qRT-PCRs were performed in triplicate on all different RNA preparations. Microarray analysis and qRT-PCR fold changes are shown for each validated gene as average values. Bars represent ± standard deviations.