but not NM
is inactivated in the presence of [ PSI
]. A. NMPM-CSC and NMCA-CSC provide translation termination function. [PSI
+] or sup35 cells ectopically expressing prion domains of either Pichia methanolica (NMPM) or Candida albicans (NMCA) fused to the C domain of Saccharomyces cerevisiae (NMSC) expressed on a URA3 plasmid on media containing low adenine-Ura and -Ade-Ura. -Ura is used to maintain the plasmid. B. NMPM-CSC is inactivated in [PSI
+] cells and not in a sup35 mutant. Around 107 [PSI
+] cells expressing either NMPM-CSC or NMCA-CSC, or sup35 mutant yeast expressing NMPM-CSC were plated on -Ade-Ura media.