Figure 4
From: Heterologous cross-seeding mimics cross-species prion conversion in a yeast model
More than one variant of [ CHI + PM ] forms in the presence of [ PSI + ]. A. Some [CHI + PM] variants have differing amounts of SDS-stable sub-particles. Lysates from [CHI + PM] colonies differing in color on low adenine media were resolved on an agarose gel and probed with an anti-HA tag antibody that detects NMPM-CSC. Stained chicken pectoralis muscle extract provided molecular weight markers. The difference in amount of SDS-stable sub-particles was reproducible for this and another pair (not shown) isolated on the basis of color difference. B. Some [CHI + PM] variants have different sized SDS-stable sub-particles. Colonies formed on -Ade-Ura could be distinguished phenotypically on low adenine media by differences in color. Lysates from these variants were resolved on an agarose gel and probed with anti-HA tag antibody, which detects NMPM-CSC. The difference in size of SDS-stable sub-particles was reproducible for this and another pair (not shown) isolated on the basis of color difference.