Figure 8

Immunofluorescence localization of AcCR29b. An antibody designed to the N-terminal region of AcCR29b was used to localize corresponding protein within epithelial cells of (a) rhinophore and (b) oral tentacle, shown in green. Arrowheads show immunopositive cell bodies and arrows point to processes exposed to the surface. Sections were counterstained with DAPI (blue) to show nuclear staining. A control in which the primary antibody was preabsorbed against its antigenic peptide showed greatly reduced staining at the same exposure (b, inset). Scale bars = 50 μm.