Figure 10

Acetylcholine acts in part through GABAergic neurons to regulate male tail posture. (a) and (b) unc-25 versus wild-type males in aldicarb: wild-type, aldicarb, N = 25; unc-25, aldicarb, N = 21; wild-type, ddH20, N = 25; unc-25, ddH20 N = 20. (a) Ventral. The highest amount of tail curling at the 20- to 25-minute time points for each trial was averaged. (b) Dorsal. The highest amount of tail curling at the 2.5- to 7.5-minute time points for each trial was averaged. (c) and (d) unc-30 versus wild-type in aldicarb. wild-type aldicarb and ddH20 N = 30; unc-30 aldicarb and ddH20 N = 29. (c) Dorsal. The highest amount at the 2.5- to 7.5-miute time points for each trial was averaged. (d) Ventral. The highest amount at 20- to 25-minute time points for each trial was averaged. (e) and (f) Mutations in the unc-49 gamma-aminobutyric acid receptor disrupt tail curling at only the 2.5-minute time point. wild-type and unc-49, aldicarb N = 30;wild-type, ddH20, N = 30; unc-49, ddH20, N = 29. (e) Dorsal tail curling at the 2.5-minute time point. (f) Ventral tail curling at the 2.5-minute time point. *P < 0.05, **P < 0.005. Fisher's exact test. Error bars indicate +/- standard error of the mean. W = water, A = aldicarb, WT = wild-type.