Figure 4
From: Effect of hydroxyurea on the promoter occupancy profiles of tumor suppressor p53 and p73
Summary of quantitative chromatin immunoprecipitation analysis of selected promoters for p53 and p73 binding before and after hydroxyurea treatment. Cross-linked chromatin from untreated hydroxyurea (-HU) or hydroxyurea-treated (+HU) (1 mM, 16 hours) HCT116-3(6) cells were immunoprecipitated with the indicated antibodies and followed by polymerase chain reaction analysis using the primers flanking the sequences corresponding to the model-based algorithm for promoter array promoter-score window in each of the promoters. Data shown are log2 occupancy units from two independent chromatin immunoprecipitation experiments +/- standard deviation. log2 occupancy units >1: significant enrichment of the selected promoter sequences in the anti-p53 or anti-p73 chromatin immunoprecipitation.