Figure 6
Expression levels of peroxisome proliferators activated receptor γ (PPARγ) mRNA in white adipose tissues of normal and diabetic mice. Total RNA was extracted from subcutaneous adipose tissue (SAT) (a) and epididymal adipose tissues (EAT) (b) of 10 week-old wild-type (WT) or db/db mice, and 20 week-old WT or diet-induced obesity mice. The mRNA expression levels of PPARγ2 were determined by real time reverse transcriptase polymerase chain reaction (RT-PCR), and normalized to that of β-actin measured in parallel as internal controls. Data represent the relative values of the mean ± SD of three independent experiments performed in triplicate.