The effect of recombinant AprA on GTP binding to membranes. The binding of [3H]GTP to membranes in the presence or absence of recombinant AprA (rAprA) was measured following . Wild-type membranes showed significantly increased [3H]GTP binding in the presence of rAprA when compared with [3H]GTP binding in the absence of rAprA with P < 0.01 (**), while gα2
- and gα9
- membranes showed increased [3H]GTP binding with P < 0.05 (*). [3H]GTP binding to gα8
- and gβ
- membranes was not significantly different (ns) (paired t-tests). Values are the mean ± s.e.m of at least three independent experiments.