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Table 3 The effect of G proteins on the mass and protein increase of cells

From: A Dictyostelium chalone uses G proteins to regulate proliferation

 

Per 107 cells per hour

Per 107 nuclei per hour

Cell type

Mass, mg

Protein, μg

Nuclei, × 10-5

Mass, mg

Protein, μg

Wild-type

0.83 ± 0.06

24 ± 1

9.9 ± 0.6

0.64 ± 0.05

19 ± 1

gα2 -

0.77 ± 0.02

24 ± 1

9.6 ± 0.4

0.57 ± 0.03

18 ± 1

gα5 -

0.99 ± 0.06

21 ± 1

12.6 ± 0.8

0.64 ± 0.05

13 ± 1

gα7 -

0.72 ± 0.06

23 ± 2

11.5 ± 1.1

0.51 ± 0.05

16 ± 2

gα8 -

1.43 ± 0.04**

37 ± 2**

15.3 ± 0.8**

0.86 ± 0.05

22 ± 2

gα9 -

1.61 ± 0.15**

42 ± 2**

12.2 ± 0.5

1.30 ± 0.13**

33 ± 2**

gβ -

1.42 ± 0.13**

42 ± 4**

16.2 ± 1.0**

0.79 ± 0.08

23 ± 3

  1. The mass, protein, and nuclei number values shown in Table 2 were divided by the observed high density doubling times from Table 1 (the mass, protein, and nuclei numbers were measured in this density range) to obtain the approximate increases in mass, protein, and nuclei per hour. All values are means ± SEM's from three or more independent assays. A ** indicates that the value was significantly different from the wild-type value with p < 0.01 (1-way ANOVA, Dunnett's test).