aFor each experimental condition, a single leaf was agro-infiltrated on 10 different Nicotiana benthamiana plants and visualized by confocal microscopy 4 days post-transfection. For each of the 10 individually infiltrated leaves, five 7-mm leaf discs were excised and 10 cells were analyzed at random from each leaf disc to avoid any bias. Thus, 500 individual cells were classified for the presence or absence of protein bodies (PBs) and for the maximum size of PBs observed within each cell.
bThese cells exhibited an endoplasmic reticulum-like pattern of fluorescence.
cPB diameter was <1.0 μm.
dPB diameter was >1.0 μm. These cells also contain small PBs.