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Figure 4 | BMC Biology

Figure 4

From: Eukaryotic protein production in designed storage organelles

Figure 4

Sf9 insect cells accumulate high levels of Zera fusions in induced PBs. (A-C) Electron micrographs of control and Zera-EGF Sf9 cells. (A) Cells immediately post-infection. (B) Electron-dense PB-like structures (PB) visible at 2 days post-infection. (C) Immunogold labeling of Zera-EGF expressing Sf9 cells using anti-EGF antisera. N: nuclei; M: mitochondria; ER. endoplasmic reticulum; PB: PB-like organelles. (D) Silver stained SDS-PAGE (left) and immunoblot (right) of protein extracts from Sf9 cells expressing Zera-EGF (lanes 2), expressing EGF alone (lanes 1) and non-infected control cells (lanes C). Note the high accumulation of Zera-EGF in lanes 2 (arrows) compared with equivalent non-Zera EGF-expressing cell extracts (lane 1, arrowhead).

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