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Table 1 Primers and probes

From: δ-Opioid receptor activation attenuates oxidative injury in the ischemic rat brain

 

Sequences of primers and probes

β-Actin

Forward primer: 5'-acggccaggtcactattg-3'

 

Reverse primer: 5'-caagaaggaaggctggaaaaga-3'

 

Probe: 5'-FAM-caacgagcggttccgatgccct-TAMRA-3'

IL1β

Forward primer: 5'-gatgaaagacggcacacc-3'

 

Reverse primer: 5'-ctatgtcccgaccattgc-3'

 

Probe: 5'-FAM-aagcggtttgtcttcaacaag-TAMRA-3'

TNFα

Forward primer: 5'-gtgttcatccgttctctaccc-3'

 

Reverse primer: 5'-gccacaattccctttctaagtt-3'

 

Probe: 5'-FAM-acccctttatcgtctactcctcagagccc-TAMRA-3'

  1. FAM = 6-carboxyfluorescin;
  2. IL = interleukin;
  3. TAMRA = 6-carboxytetramethylrhodamine;
  4. TNF = tumor necrosis factor.