The ALPS motif of GMAP210 is sufficient for Golgi binding. (A, B) Schematic representation of the GFP-tagged N-terminal domain of GMAP210 and all the truncated or fusion constructs in this domain that were tested for their binding to GA (A) and their migration on SDS-PAGE as revealed by Western blot with an anti-GFP antibody after expression in RPE1 cells (B). The ALPS-like motif is represented in red and coiled-coil regions in light blue. The yellow rectangle corresponds to the continuous coiled-coil domain used in the indicated constructs. (C to J) Subcellular localisation of the different N-terminal mutants and other constructs (as indicated) expressed in RPE1 cells and double labelled for GM130. Arrows in (F1) indicate ERES. Bars = 10 μm.