A detailed analysis of the distribution of the ALPS-CC mutant protein in Golgi membranes. (A, B) Cells transfected with the ALPS-CC-GFP construct (A) were treated (B) with nocodazole and labelled with anti-GM130 and anti-Golgin245 antibodies. Merged images are shown. Enlarged views of insets show merged images of GFP and Golgin245 labellings in left panels, merged images of GM130 and Golgin245 labellings in middle panels and merged images of the three labellings in right panels. At the bottom, fluorescence intensity profiles of lines drawn over Golgi membranes revealed cis-Golgi localisation of the mutant (lane 1) but also a striking enrichment in regions from which GM130 is excluded and vice versa (arrows and lanes 2 and 3). Bars = 5 μm.