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Table 1 Structure and ecoregion cluster distribution of the A. marginale MSP1a microsatellites.

From: Phylogeographic analysis reveals association of tick-borne pathogen, Anaplasma marginale, MSP1a sequences with ecological traits affecting tick vector performance

Genotype

Number of strains

m

n

SD-ATG distance (nucleotides)

Genotype frequency per ecoregion cluster

     

1

2

3

4

A

2

1

7

19

0.00

1.00

0.00

0.00

B

5

1

9

23

1.00

0.00

0.00

0.00

C

7

2

5

19

0.57

0.14

0.29

0.00

D

3

2

6

21

0.33

0.67

0.00

0.00

E

12

2

7

23

0.75

0.00

0.25

0.00

F

3

3

4

21

0.00

0.00

1.00

0.00

G

78

3

5

23

0.15

0.14

0.56

0.14

H

3

3

6

25

0.00

0.67

0.33

0.00

I

NI

4

6

29

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  1. The MSP1a microsatellite sequences were analyzed in 115 A. marginale strains. The microsatellite (sequence in bold) was located between the Shine-Dalgarno (SD; sequence in brackets) and the translation initiation codon (ATG) with the structure: GTAGG (G/A TTT) m (GT) nT ATG. The SD-ATG distance was calculated in nucleotides as (4 × m) + (2 × n) + 1. Abbreviation: NI, not included in the study because the A. marginale strain was not adequately geo-referenced.