|
cbh1SF
|
5'-TCGGCCTGCACTCTCCAATCC-3'
|
PCR fragments used as probes for northern blots
|
|
cbh1SR
|
5'-TGGAGTCCAGCCACAGCATG-3'
| |
|
18SRF
|
5'-GGTGGAGTGATTTGTCTG-3'
| |
|
18SRR
|
5'-CTTACTAGGGATTCCTCG-3'
| |
|
hfb1F
|
5'-CTGAACACCTCCAGTCAAC-3'
| |
|
hfb1R
|
5'-TATTGGAGAATAAGAATGGC-3'
| |
|
hfb2F
|
5'-TCAAGATGCAGTTCTTCG-3'
| |
|
hfb2R
|
5'-CATTGCTTTAGAAGGTGC-3'
| |
|
gna1aa5F
|
5'-ACACGAACGCCCAAATCTG-3'
|
Construction of vector pBgna1Q204L
|
|
gna1aa5R
|
5'-CTTTCGCTCAGATCGCTG-3'
| |
|
gna1aa3F
|
5'-GCCAGCGATCTGAGCGAAAGAAG-3'
| |
|
gna1aa3R
|
5'-GGGCGACGGCACATTAACATAG-3'
| |
|
gna1aa5NF
|
5'-atgtcgacCAAATCTGCGTCTTCCACAC-3'
| |
|
gna1aa3NR
|
5'-atgggcccCGGCACATTAACATAGCTTG-3'
| |
|
gna1F
|
5'-TAAGCGAAGGTTCTCCTG-3'
|
cDNA amplification
|
|
gna1R
|
5'-ATTGGCATTTCATTCCTC-3'
| |
|
gna1D5F
|
5'-atgggcccCATCTCCACAGTTTCCAGG-3'
|
Construction of vector pDELgna1
|
|
gna1D5R
|
5'-atgtcgacTGTTGGCGACTGTGAATG-3'
| |
|
gna1D3F
|
5'-atggatccTTAGACGACTTGTTGATTGTTG-3'
| |
|
gna1D3R
|
5'-attctagaCCCAATGCTTATAGAACATC-3'
| |
