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Table 1 Oligonucleotides used in this study

From: Light-dependent roles of the G-protein α subunit GNA1 of Hypocrea jecorina (anamorph Trichoderma reesei)

Name Sequence Application
cbh1SF 5'-TCGGCCTGCACTCTCCAATCC-3' PCR fragments used as probes for northern blots
cbh1SR 5'-TGGAGTCCAGCCACAGCATG-3'  
18SRF 5'-GGTGGAGTGATTTGTCTG-3'  
18SRR 5'-CTTACTAGGGATTCCTCG-3'  
hfb1F 5'-CTGAACACCTCCAGTCAAC-3'  
hfb1R 5'-TATTGGAGAATAAGAATGGC-3'  
hfb2F 5'-TCAAGATGCAGTTCTTCG-3'  
hfb2R 5'-CATTGCTTTAGAAGGTGC-3'  
gna1aa5F 5'-ACACGAACGCCCAAATCTG-3' Construction of vector pBgna1Q204L
gna1aa5R 5'-CTTTCGCTCAGATCGCTG-3'  
gna1aa3F 5'-GCCAGCGATCTGAGCGAAAGAAG-3'  
gna1aa3R 5'-GGGCGACGGCACATTAACATAG-3'  
gna1aa5NF 5'-atgtcgacCAAATCTGCGTCTTCCACAC-3'  
gna1aa3NR 5'-atgggcccCGGCACATTAACATAGCTTG-3'  
gna1F 5'-TAAGCGAAGGTTCTCCTG-3' cDNA amplification
gna1R 5'-ATTGGCATTTCATTCCTC-3'  
gna1D5F 5'-atgggcccCATCTCCACAGTTTCCAGG-3' Construction of vector pDELgna1
gna1D5R 5'-atgtcgacTGTTGGCGACTGTGAATG-3'  
gna1D3F 5'-atggatccTTAGACGACTTGTTGATTGTTG-3'  
gna1D3R 5'-attctagaCCCAATGCTTATAGAACATC-3'  
  1. Mutated bases are given in bold, artificially introduced restriction sites are given in lower case letters.
  2. PCR = polymerase chain reaction.