Figure 4
From: Receptor oligomerization and beyond: a case study in bone morphogenetic proteins
Binding of radiolabeled ligands using higher ligand concentrations. (a) Specific binding of core bone morphogenic protein (wild type) (coreBMP2wt) to BMP receptor (BMPR)-IA transfected results in a biphasic binding curve with a pronounced break (marked by arrow). Fitting the curve separately (0 to 500 pM and 1,000 to 4,000 pM) to a one-site binding model the indicated values for K D and B max were achieved. (b) Specific binding of coreBMP2wt to COS-7 cells transfected with activin receptor (ActR)-IIB and (c) to untransfected C2C12 cells.