Figure 4

RNAi knockdown of MMR-A7 and MMR-B3 genes and comparison of their effects on male mating behavior. All transfections are compared with a negative control transfected with phosphate-buffered saline instead of double-stranded RNA (dsRNA). AB was transfected with a half dose of MMR-A7 plus a half dose of MMR-B3. MAN was transfected with dsRNA from the GDP-mannose 4,6-dehydratase gene. Percent circling is the number of circling behaviors performed by males divided by the number of male-female encounters. Asterisks indicate significant reductions in male circling; percentages with downward arrows indicate the magnitude of the reduction. Vertical lines on top of each column represent standard errors.