Expression of ClantHox in embryos and juveniles of C. longifissura. (a) ClantHox expression in animal/anterior ectodermal cells in the postgastrula. (b) Bilateral domains of ClantHox expression located more internally in the animal hemisphere of the embryo during later development. (c) Double in situ hybridization of ClantHox (purple) with ClSix3/6 (turquoise), black arrows indicate the two ClantHox expression domains in the animal hemisphere. ClSix3/6 is found anterior to the ClantHox domain. Note that the ClSix3/6 staining is less localized than in (d) due to diffusion of the dye when using only 5-bromo-4chloro-3-indolyl-phosphate (BCIP) as substrate. (d) ClSix3/6 expression in the animal/anterior hemisphere. (e) Co-localization of the expression of ClantHox (area shown by a dotted line) with ClSoxB1 expression in the late postgastrula of C. longifissura both visualized with BCIP/nitro blue tetrazolium chloride (NBT). (f) ClSoxB1 expression clone of the same stage as (e). (g) Dorsal view on hatched juvenile showing expression of ClantHox. White arrow points at the higher expressing area in the post-statocyst commissure. (h) Lateral view in a hatched juvenile of C. longifissura with expression of ClantHox in the ectoderm and parenchyma. Arrow points at domain of commissural expression. (i) Summary of ClantHox expression is shown in orange, dorsal view, (j) lateral view. st = statocyst, ey = eye, mo = mouth opening. (a - f) Animal/anterior pole up; (g - j) anterior to the left.