Table 3 Primers used for reverse transcription polymerase chain reaction analysis and sequence confirmation.
From: TonB-dependent transporters and their occurrence in cyanobacteria
Use | Primer | Oligonucleotide sequence |
|---|---|---|
RT-PCR | all1101-F | CGCTGCTCTATCGCCTGACTG |
| Â | all1101-R | GCGTACTTTCCAGCGATTGTGC |
| Â | all2148-F | ACGGTGACGGGGGAAACAGGA |
| Â | all2148-R | ACTTCCACTCGCTCAATCGTCC |
| Â | all2158-F | CACCACCAGCAGAACCAACAGC |
| Â | all2158-R | CGATAAATCCACCTCACCACGG |
| Â | all2236-F | AATCGCGCGGCACTCTACCGT |
| Â | all2236-R | GGATAACTCCAATCCCACGAGC |
| Â | all2610-F | CAGTCATAGGAGAGGCGGGATT |
| Â | all2610-R | CATAGAGTACAGAAGCCGGTCC |
| Â | all2620-F | AAACTCCCTCAACCGCGCTGG |
| Â | all2620-R | GGAATCCGCGAACCATCGGC |
| Â | all2674-F | CCCAGAAACACCTACCGCAGAA |
| Â | all2674-R | AACTAGGTTGACGACCCCACCT |
| Â | all3310-F | TTTAGGCAACCCAGGCGGCAC |
| Â | all3310-R | GCATTATGCTCAAAGGTGACGCG |
| Â | all4026-F | GTGGTTTTTGTGGAGTGTGGGG |
| Â | all4026-R | CCATCAACTGGTGTGTCTTCCC |
| Â | all4924-F | CCCTACCAGAGGATCTGGGGA |
| Â | all4924-R | CTGTACCAACGGCTGGTAAGAC |
| Â | alr0397-F | TGCGTCGCGGGATTTGCGAAC |
| Â | alr0397-R | GGATAGTATTGACCCTGGGGTC |
| Â | alr2153-F | CCTCCCGTGGGATTAACTTTGG |
| Â | alr2153-R | CGCATCAGGGCCCACTCGA |
| Â | alr2175-F | GGTGTCCCGGCTGTTGGTACT |
| Â | alr2175-R | AGTACCTCAAACCTCTCTGGGC |
| Â | alr2185-F | CCCCGTCAGGTACTCGAAGAC |
| Â | alr2185-R | TCTTCCATGTCAAGCTAGGGGC |
| Â | alr2209-F | CCTACTCCCACACCCCCAAC |
| Â | alr2209-R | CTGTCTTGGTCAGGTCTTCGGG |
| Â | alr2211-F | CAAGATCGCCAAGTGGTGAGGC |
| Â | alr2211-R | GTGTTTCCAGGTAACAAGCCCC |
| Â | alr2581-F | GCGGGGACAGAAGGCAAATTTG |
| Â | alr2581-R | CGCCTAATTGTTCTGTACCCCC |
| Â | alr2588-F | CAGGTGAGGCGGGATTACCTG |
| Â | alr2588-R | ACTCCCCCTGGTTCTAGTTGTC |
| Â | alr2592-F | CCCCAAAGCCAGTGGAGGGA |
| Â | alr2592-R | CCCCTAGCGGCTCGAACATTG |
| Â | alr2596-F | CGCCTGTGCGCGATATTCCTG |
| Â | alr2596-R | CAGGCGCAACAAATACCCGTTC |
| Â | alr2626-F | GGCGTTCAACCAGGGGGAGT |
| Â | alr2626-R | CTAAACCAGGTCTGGGTATGGC |
| Â | alr3242-F | CCCGACGTGATAGTGGGTCAC |
| Â | alr3242-R | CTGGGGGAATCCGGCTGCAT |
| Â | rnpB-F | AGGGAGAGAGTAGG GTTGG |
| Â | rnpB-R | GGTTTACCGAGCCAGTACCTCT |
Sequence confirmation | all2619-20 F | CTCCCATTTCTCCGAAGCTG |
| Â | all2619-20 R | CAACGCTGGGGCCAACATAG |
| Â | alr4028-29 F | CTATGGACTTAACCAACAAAGCATTC |
| Â | alr4028-29 R | CTTCTCTGGTTTAAGGTCAGGATTAC |