Analysis of cuticular hydrocarbon (CHC) profiles using gas chromatography-mass spectrometry. (A) Example chromatogram from control (colonymate CHC-treated) ants. (B) Example chromatogram from ants treated with a combination of the two synthetic single-methyl C35s (arrow). (C) Example chromatogram from ants treated with a combination of the three synthetic single-methyl C37s (arrow). Because a mixture of the synthetic CHCs was applied, the effective concentration of each individual CHC is a half and one-third of the peak size shown here, respectively.