Table 1 Summary of recovery of pyrosequencing tags for Framvaren (FV) and Cariaco (CAR) samples, along with accompanying metadata.
From: Massively parallel tag sequencing reveals the complexity of anaerobic marine protistan communities
| Â | Sample number-sample name | |||||||
|---|---|---|---|---|---|---|---|---|
| Â | 1-FV1 | 2-FV2 | 3-FV3 | 4-FV4 | 5-CAR1 | 6-CAR2 | 7-CAR3 | 8-CAR4 |
N 454-reads total | 38735 | 34171 | 24217 | 33962 | 35267 | 30277 | 28305 | 26714 |
Total eukaryotic tags > 100 bp | 38280 | 32026 | 16256 | 32795 | 32876 | 22503 | 24266 | 23591 |
Unique eukaryotic tags | 4280 | 5283 | 3765 | 5141 | 5983 | 5701 | 4325 | 4016 |
Total protistan tags (incl. Fungi) | 23722 | 29402 | 12864 | 26543 | 30161 | 14453 | 7166 | 5969 |
Unique protistan tags (incl. Fungi) | 3220 | 4825 | 3204 | 4439 | 5597 | 4616 | 2152 | 2070 |
Total and (unique) unassignable tags at 85% | 1338 | 1153 | 1178 | 9189 | 2255 | 1724 | 1768 | 1042 |
| Â | (276) | (468) | (427) | (758) | (620) | (580) | (556) | (365) |
Total and (Unique) Archaeal tags | 0 (0) | 2 (2) | 2(1) | 2 (2) | 0 (0) | 4 (3) | 6 (5) | 2 (2) |
Total and (Unique) Bacterial tags | 0 (0) | 0 (0) | 1 (1) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
Latitude/Longitude | 58°09'N | 58°09'N | 58°11'N | 58°09'N | 10°30'N | 10°30'N | 10°40'N | 10°30'N |
|  | 06°45'E | 06°45'E | 06°45'E | 06°45'E | 64°40'W | 64°40'W | 65°35'W | 64°40'W |
Temperature °C | 10.7 | 8.4 | 8.1 | 5.8 | 17.9 | 17.7 | 17.6 | 17.6 |
Depth (m) | 20 | 36 | 36 | 36 | 250 | 300 | 320 | 300 |
Salinity [80] | 27 | 28 | 27.5 | 25.5 | 36.4 | 36.4 | 36.4 | 36.4 |
Nitrate (μmol/l) | --- | --- | --- | --- | 5.22 | nd | nd | 0.02 |
Silicate (μmol/l) | --- | --- | --- | 100 | 31 | 39 | 41 | 43 |
Ammonium (μmol/l) | 0.22 | 2.2 | --- | 2.2 | 0.12 | 1.27 | 2.4 | 3.2 |
O2 (μmol/l) | nd | nd | nd | nd | nd | nd | nd | nd |
H2S (μmol/l) | nd | 668 | 362 | 600 | nd | 1.49 | 3.74 | 4.28 |
Bacteria (× 106 cells/ml) | 4.6 | 0.78 | 0.43 | 0.61 | 0.487 | 0.149 | 0.18 | 0.244 |
Bact Production (H3-Leu, mg/m2/d) | 670 | 160 | ---- | ---- | 347 | 353 | 1305 | 61 |
Chlorophyll a (μg/l) | 1.22 | nd | nd | nd | nd | nd | nd | nd |
DNA conc (ng/μl) | 160 | 170 | 170 | 120 | 5.58 | 4.55 | 9.12 | 10.34 |
Water volume sampled (/) | 15 | 20 | 20 | 20 | 7 | 7 | 7 | 5 |
Sampling date | Sept-2005 | Sept-2005 | Sept-2005 | May-2004 | Jan-2005 | Jan-2005 | Jan-2005 | May-2005 |