Figure 2

Examples for enhancer traps and mutant phenotypes. Enhancer traps (A-H) and mutant phenotypes (I-L) of piggyBac insertion lines. For details on the potentially affected genes, see methods part "Location of insertion lines shown in Figure 2". (A) EGFP expression in the cuticle during all larval stages. (B) Pupa showing EGFP expression in a subset of somatic muscles. (C) Embryo showing EGFP expression in the abdomen. (D) Embryo showing EGFP expression in two lateral stripes, which based on the similarity to the Drosophila expression pattern of lame duck is presumably located in the mesoderm. (E) Pupa showing EGFP in a "salt and pepper" pattern in the ventral abdominal epidermis. (F) Embryos showing EGFP expression in the distal legs. (G) Embryos showing EGFP expression in the hindgut and in segmental stripes. (H) EGFP expression is in the proximal embryonic leg, as well as in T2, T3, and A1 spots, and a posterior ring in the first-instar larva. (I) Homozygous embryo is poorly differentiated and has bubbly short legs and segmental defects. (J) Maxillary (grey arrows) and labial (white arrow) palps are transformed to legs while the overall morphology of the segments appears unchanged (this corresponds to the described Tc-maxillopedia mutant phenotype [16, 17]). (K) Homozygous embryonic cuticle showing dorsal defects and possibly additional patterning or differentiation problems. (L) Homozygous embryo with rudimentary appendages in the first abdominal segment which also lacks tracheal openings.