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Table 1 Results of lethality/sterility test (F3 cross)

From: Large-scale insertional mutagenesis of a coleopteran stored grain pest, the red flour beetle Tribolium castaneum, identifies embryonic lethal mutations and enhancer traps

First round of single-pair matings Second round of single-pair matings
phenotype number of insertions phenotype number of insertions
viable* 4908 (86.8%) viable 250 (4.4%)
potentially lethal 589 (10.4%) lethal 421 (7.4%)
potentially sterile 160 (2.8%) sterile 8 (0.1%)
   not retested 70 (1.2%)
  1. A total of 5,657 lines were tested for potential lethality or sterility by a first round of single-pair matings (left half of table; see Results and Methods for details). Those that matched the criteria (749) were retested by a second round of additional single-pair matings in order to eliminate false positives (right half of table). Only those lines that matched the definition in the second round were considered to be lethal or sterile. All percentages are calculated based on the original total of 5,657 lines.
  2. *This number includes 236 lines that were considered potentially semi-lethal (see text for definition of semi-lethality). Because this was done only on a subset of 2,940 lines, the numbers are not given separately.