First round of F3 single-pair matings (SPM) | Second round of F 3 single-pair matings (SPM) | ||
---|---|---|---|
Offspring | Phenotype | Offspring | Phenotype |
At least one SPM indicates homozygosity | Viable | At least one SPM (in total) indicates homozygosity | Viable |
All informative* SPM indicate heterozygosity of both parents | Potentially lethal | All informative* SPM indicate heterozygosity of both parents | Lethal |
At least two SPM without any offspring but with living parents | Potentially sterile | Unable to find at least four SPM (in total) without any offspring but with living parents (method 1) OR unable to identify either a fertile homozygous female or a fertile homozygous male (method 2) | Sterile |