Figure 1 From: Predominant membrane localization is an essential feature of the bacterial signal recognition particle receptor Binding of FtsY to ribosome nascent chains requires the presence of signal recognition particles (SRP) and guanosine 5'( β ,-γ imido) triphosphate (GMP)-PNP. MtlA189 RNCs were in vitro synthesized in the presence of purified SRP and in vitro synthesized, purified FtsY. When indicated puromycin or GMP-PNP were added. Ribosome-nascent chains (RNCs) were subsequently separated by centrifugation through a sucrose cushion into pellet fraction (P) and supernatant (S), which were analysed on SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Both panels of the figure correspond to the same gel but were separated due to the large size-difference between FtsY and the RNCs. The radioactive material was quantified using a phosphor imager and the Imagequant software. Three independent experiments were performed.Back to article page