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Figure 4 | BMC Biology

Figure 4

From: Factors necessary to produce basoapical polarity in human glandular epithelium formed in conventional and high-throughput three-dimensional culture: example of the breast epithelium

Figure 4

HTP culture produces a basoapical polarity axis similar to that shown with classical 3D-Matrigel™ cultures. A. Organization of the basoapical polarity axis in 2D and HTP cultures of S1 cells as shown by immmunofluorescence staining of basal (collagen IV [green], α6-integrin [green]), lateral [beta-catenin [red] and desmoplakin 1,2 [red]), lateroapical [ZO-1 [green]), and apical (mucin-1 [green]) polarity markers. Nuclei are counterstained with DAPI (blue). Size bar, 5 μm. B. drawings of the different cell culture systems used in this study (2D monolayer; Matrigel™-embedded: cells totally embedded in the Matrigel™ gel; 3D-Matrigel™ drip: 5% Matrigel™ drip on Matrigel™ gel-coated surface; HTP: 5% Matrigel™ drip on glass surface). The gel is represented in red; soluble Matrigel™ dripped in the culture medium is represented by curvy lines.

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BMC Biology

ISSN: 1741-7007

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