Composition | H14 medium | Assay medium |
---|---|---|
Type of medium | DMEM/F12 | DMEM/F12 |
Prolactin | 5 μg/ml | - |
Insulin | 250 ng/ml | 10 μg/ml |
Hydrocortisone | 1.4 μg/ml | 0.5 μg/ml |
17-Beta-estradiol | 10-10 M | - |
Sodium Selenite | 2.6 ng/ml | - |
Transferrin | 10 μg/ml | - |
EGF | 10 ng/ml | 5 ng/ml |
Horse serum | - | 2% |
Cholera toxin | - | 100 ng/ml |
Antibiotics | - | 50 U/ml penicillin 50 μg/ml streptomycin |
% of Matrigelâ„¢ drip for 3D culture | 5% | 2% |
2D culture process for passages | culture for 8 to 12 days | culture for 3 to 4 days Medium different from 3D culture medium (5% horse serum, 20 ng/ml EGF) |