DMXAA treatment of TNF-α targeted and non-targeted R-Luc reporter cell lines. (a) Relative R-Luc activity in the targeted (Tg) and non-targeted (nTg #1–4) reporter cell lines in the absence or presence of DMXAA (100 μg/ml). (b) Dose-response profile for R-Luc activity following treatment of the Tg and nTg4 cell lines with DMXAA. (c) Dose-response profile for DMXAA-induced cytotoxicity in Tg and nTg4 cell lines. (d) Induction of TNF-α mRNA in the Tg and nTg4 cell lines following treatment with DMXAA. Despite the fact that DMXAA only induces R-Luc reporter activity in the Tg cell line, DMXAA induced TNF-α mRNA to equivalent levels in both cell lines. Values represent the mean (+/-SEM, N = 4).