Ecotropic viral integrative factor 5 (EVI5) network. Network of direct interactions of six proteins with EVI5. Green = positive regulation; orange = negative regulation. The 110-kDa form of EVI5 stabilizes Emi1 to prevent premature entry into mitosis. At the same time, EVI5 inhibits the vesicle trafficking function of Rab 11a and b. Polo-like kinase 1 (PLK1) is then activated by inner centromere protein (INCENP) to degrade both EVI5 and Emi1, allowing progression into mitosis (M). During M, 90-kDA and 20-kDa forms of EVI5 interact with the chromosomal passenger complex (CPC) proteins aurora B kinase, INCENP, and survivin, where EVI5 is necessary for cytokinesis.