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Table 3 Liquid chromatography/mass spectrometry/mass spectrometry (LC/MS/MS) versus densitometry measurements

From: Proteomic analysis of blastema formation in regenerating axolotl limbs

Protein Comparison Fold change
   LC-MS/MS Densitometry
NOS1 0 dpa vs 1 dpa 4.93 2.0*
  0 dpa vs 7 dpa 1.16 1.2***
Fibronectin 0 dpa vs 1 dpa 1.39 1.51**
  0 dpa vs 7 dpa 1.46 3.17*
α-Actinin 0 dpa vs 1 dpa -1.16 -0.62***
  0 dpa vs 7 dpa -1.58 -0.83
  1. Comparison of fold changes for NOS1, fibronectin and α-actinin with densitometry measurements on immunostained sections. Six images were collected for each immunostained section. Mean pixel intensities were calculated for each image by sampling 20 randomly-distributed regions of each image using the measurement package of the Axiovision software (Zeiss). Statistical comparisons were performed using ANOVA. A P value < 0.05 was considered statistically significant.
  2. *P < 0.001; **P < 0.01; ***P < 0.05.
  3. ANOVA = analysis of variance; dpa = days post amputation; NOS1 = nitric oxide synthase 1.
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