Comparison of fold changes for NOS1, fibronectin and α-actinin with densitometry measurements on immunostained sections. Six images were collected for each immunostained section. Mean pixel intensities were calculated for each image by sampling 20 randomly-distributed regions of each image using the measurement package of the Axiovision software (Zeiss). Statistical comparisons were performed using ANOVA. A P value < 0.05 was considered statistically significant.
*P < 0.001; **P < 0.01; ***P < 0.05.
ANOVA = analysis of variance; dpa = days post amputation; NOS1 = nitric oxide synthase 1.