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Table 3 Liquid chromatography/mass spectrometry/mass spectrometry (LC/MS/MS) versus densitometry measurements

From: Proteomic analysis of blastema formation in regenerating axolotl limbs

Protein

Comparison

Fold change

  

LC-MS/MS

Densitometry

NOS1

0 dpa vs 1 dpa

4.93

2.0*

 

0 dpa vs 7 dpa

1.16

1.2***

Fibronectin

0 dpa vs 1 dpa

1.39

1.51**

 

0 dpa vs 7 dpa

1.46

3.17*

α-Actinin

0 dpa vs 1 dpa

-1.16

-0.62***

 

0 dpa vs 7 dpa

-1.58

-0.83

  1. Comparison of fold changes for NOS1, fibronectin and α-actinin with densitometry measurements on immunostained sections. Six images were collected for each immunostained section. Mean pixel intensities were calculated for each image by sampling 20 randomly-distributed regions of each image using the measurement package of the Axiovision software (Zeiss). Statistical comparisons were performed using ANOVA. A P value < 0.05 was considered statistically significant.
  2. *P < 0.001; **P < 0.01; ***P < 0.05.
  3. ANOVA = analysis of variance; dpa = days post amputation; NOS1 = nitric oxide synthase 1.
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BMC Biology

ISSN: 1741-7007

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