THOC5/FMIP deletion causes reduction of spleen size and induction of cleaved caspase 3. Two hundred and fifty micrograms of poly (I:C) were injected into six-week-old Mx-cre THOC5/FMIP (flox/flox) (n = 9) or control THOC5/FMIP (flox/flox) (n = 6) mice three times at two-to-three-day intervals. (A) Weight of body, liver, spleen and heart. (B) Macrography of liver and spleen: seven days after the first poly (I:C) treatment. Mx-cre -: control mice (THOC5/FMIP (flox/flox)); Mx-cre +: THOC5/FMIP depleted mice (Mx-cre THOC5/FMIP (flox/flox)). (C, D) Seven days after the first poly (I:C) injection (3×), mouse spleens, heart, liver were fixed in formalin. Paraffin sections were stained by hematoxylin and eosin (C). Spleen and liver sections were supplied for immunohitochemical staining using cleaved caspase 3 (D). Original magnification: ×100 for C and ×200 for D.