Period of Darkness Before Illumination
(min)
|
Spheroids in Area 1*
(%)
|
SD
(%)
|
---|
0
|
100
|
2.5
|
0-10
|
97.3
|
3.1
|
10-20
|
86.5
|
8.7
|
20-30
|
87.7
|
3.3
|
30-40
|
84.5
|
1.3
|
40-50
|
81.5
|
4.1
|
50-60
|
67.2
|
11.7
|
60-70
|
65.2
|
6.1
|
70-80
|
63.8
|
11.1
|
80-90
|
61.9
|
13.6
|
- *The duration of the photoaccumulation assay was 30 s (n = 3 for each time period). The number of light-adapted spheroids accumulated in area 1 (setup B, Figure 3b) was set to 100%. These light-adapted spheroids correspond to 0 min in the dark. The light intensity during both the light adaptation and the photoaccumulation assay was 2.08 μmol m-2 s-1.