A mixed community of actinomycetes produce multiple antibiotics for the fungus farming ant Acromyrmex octospinosus

Table 1 Nystatin P1 biosynthetic genes.

Contig ID	Pseudonocardiasp. P1 protein	Proposed function*	P. autotrophicaortholog	Identity (%)
PP100949	NypF	Phosphopantetheinyl transferase	NppF	89
PP100949	NypY	Glycosyltransferase	None†	--
PP100949	NypZ	Metallophosphoesterase	None‡	95
PP100398	NypH	ABC transporter	NppH	88
PP100398	NypDIII	dGDP-mannose-4,6-dehydratase	NppDIII	96
PP100400	NypL	P450 monooxygenase	NppL	84
PP100400	NypN	P450 monooxygenase	NppN	94
PP100400	NypDII	Aminotransferase	NppDII	96
PP100400	NypDI	Glycosyltransferase	NppDI	92
PP100821	NypE	Thioesterase	NppE	92
PP100306	NypO	Acyl-CoA decarboxylase	NppO	96
PP100306	NypRIV	LuxR transcriptional regulator	NppRIV	93
PP100306	NypM	Hypothetical protein	NppM§	82

*Proposed function of full length nystatin P1 biosynthetic (nyp) genes present in the draft genome of Pseudonocardia sp. P1 (Genbank accession ADUJ00000000).
† NypY is a glycosyltransferase unique to the nystatin P1 biosynthetic gene cluster and is not orthologous to proteins in the nystatin-like Pseudonocardia polyene (NPP) biosynthetic gene cluster from P. autotrophica (AC = EU108007) or the nystatin biosynthetic gene cluster from Streptomyces nouresi (AC = AF263912).
‡ The nystatin P1 and NPP biosynthetic gene clusters contain a putative metallophosphoesterase downstream of nypH and nppH, respectively that is not present in the nystatin biosynthetic gene cluster from S. nouresi. This open reading frame was not originally annotated by Kim et al. [15] and we have therefore given the Pseudonocardia P1 ortholog the name of nypZ.
§ nypM encodes a hypothetical protein with high homology to NppM, which is annotated as a putative ferredoxin [15], however amino acid homology-based database searches failed to reveal homology to ferredoxin proteins.

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