Single locus affects embryonic segment polarity and multiple aspects of an adult evolutionary novelty

Table 1 Segregation of aberrant embryos and adults in experimental crosses

Cross	No. fam	No. progeny	Aberrant embryos			No. adult progeny with eyespot phenotypes
			% [SD]	χ²_HOM[P]	χ²_GF[P]	WT	BE	Fr	Spr	χ²_HOM[P]	χ²_GF[P]
BE × BE	16	2954	24.0 [2.9]	12.89 [0.6107]	1.68 [0.1949]	250	521	0	0	3.70 [0.9986]	0.28 [0.5967]
Fr × Fr	10	1156	27.4 [3.1]	4.98 [0.8373]	3.61 [0.0574]	175	0	334	0	3.38 [0.9473]	0.25 [0.6171]
Spr × Spr	13	1416	26.4 [4.3]	8.97 [0.7065]	1.51 [0.2191]	0	153	0	287	4.60 [0.9700]	0.41 [0.5220]
BE² × BE²	8	1488	22.9 [2.2]	4.03 [0.7765]	3.44 [0.0636]	151	278	0	0	13.99 [0.0514]	0.67 [0.4131]
BE³ × BE³	4	667	3.1 [2.3]	10.78 [0.0292]	-	0	393	0	0	-	-
BE × WT	14	2459	0	-	-	499	549	0	0	18.58 [0.1367]	2.39 [0.1221]
Fr × WT	5	659	0	-	-	227	0	249	0	0.69 [0.9526]	1.02 [0.3125]
Spr × WT	9	1272	0	-	-	275	314	0	0	4.68 [0.8613]	2.59 [0.1075]
BE³ × WT	11	-	-	-	-	1486	0	0	0	-	-
BE × Fr	9	1671	24.4 [4.1]	10.24 [0.2486]	0.31 [0.5777]	185	185	164	0	-	-
BE × Spr	24	2974	23.6 [4.8]	27.26 [0.2451]	3.05 [0.0807]	408	480	0	412	-	-
BE³ × BE	7	1092	1.0 [4.0]	90.98 [0.0000]	-	356	0	0	310	-	-

The ratios of aberrant embryos and adults were tested for variation among families within each cross with χ² test for homogeneity (χ² _HOM with d.f. = N_fam - 1). When ratios were not significantly different across families, the numbers of progeny were pooled and tested against the expected ratios with χ² goodness-of-fit test (χ² _GF with d.f. = 1). Otherwise, the ratios were tested against the expected values in each family separately (d.f. = 1; see "Different BE phenotypes and variation in lethality of underlying alleles" section). Standard deviation (SD) and P values (P) are given in square brackets.